Pukupuku katsurai (Muramoto, 2002)

The species was described by Mr. Masayuki Fujioka under his wife's name Mrs. Rieko Muramoto in 2002. In the original description, the new species belong to Fruhstorferia genus, however the author then erected a new genus and moved two related species Frustorferia curta and F. katsurai to Pukupuku.
The species was named after Mr. Katsura who collected insects from Vietnam in many years, he married a Vietnamese girl, her name is Doan Lan Giang.
Pukupuku katsurai is a very particular species and the mature form flies in a short duration of beginning of spring in North Vietnam so it is quite rare. The species recently also found from South China.
In Vietnam, only a locality was recorded as the habitat of the species, the pristine forest, Pia Oac Nature Reserve, Cao Bang Province, North Vietnam where also is type locality of the species.

Pukupuku katsurai (Muramoto, 2002) - Male in dorsal view

Pukupuku katsurai (Muramoto, 2002) - Male in lateral view

How to access the male genitalia of a scarab beetle for studying in the laboratory

Genitalia analyze is one of very important step of studying taxonomy of insects in general and beetles particularly. Not only because they are very variable in mature form but also they are very similar among individuals that not belong same species. For some group of beetles, genitalia is not the key characteristic for separate species but in some group, the male genitalia is only way to identify them to species level.

Each taxonomist developed himself a way to study his group, this posting I am going to express how I access the male genitalia of a horned rutelinae for studying without cut them off from the specimen.

Prepare a hot water cup/box for the dried specimen that collected from the field. 

 Keep the male beetle into the hot water, duration up to how dried specimen is and with hotter water, the specimen will be flexible in a shorter time (do not reboil water with the beetle in because too high temperature will destroys the pigment chemical agents (color).

From left to right (below): box of hot water (now maybe warm water) with beetle specimen, soft tissue paper (toilet paper, don't apply an used toilet paper in this case, we need a clean one), foam board; above: forceps and insect pins.

Make the beetle dried by soft tissue paper, both side of the specimen.

Transfer to the foam board for fix the poission

Fixed specimen with entomological pins then you can access the last abdomen segment without moving

Prepare 2 forceps with point tips, each hand with each one.

 Transfer the beetle to a microscope and see detail the last segment of the abdomen.

Use the point forceps open the last segment

Then you can see the apex of the penis

Then try to pull out the penis from the abdomen

Do it slowly without damage the penis apex (it very sensitive and easy to be broken)

Release specimen by removing the insect pins


Open the mandible make them separate in a good view poisition


Turn the specimen to express position


Fix the specimen in dorsal position with insect pins again.

Under microscopic field, check the shape and position of the genitalia in dorsal view. Shape of the genitalia shows that it look like Kibakoganea dohertyi

Dried fixed specimen in a box with silica-gel in side

As your specimen full dried up, it will ready for pinning or gluing on the card board, do not forget the data label of the specimen. 

Checklist of Rutelinae, Scarabaeidae of Vietnam

1. Adoretosoma chromaticum Fairmaire, 1886
2. Adoretosoma elegans Blanchard, 1851
3. Adoretosoma nigripenne Ohaus, 1905
4. Adoretus (Chaetadoretus) alocopygus Ohaus, 1914
5. Adoretus (Chaetadoretus) kulzeri (Frey, 1970)
6. Adoretus (Chaetadoretus) vitalisi Ohaus, 1930
7. Adoretus (Lepadoretus) fuscoseriatus Ohaus, 1914
8. Adoretus (Lepadoretus) griseosetosus Nonfried, 1891
9. Adoretus (Lepadoretus) popei (Frey, 1970)
10. Adoretus (probably s. str.) baquari Abdullah & Roohi, 1968
11. Adoretus (s str) cochinchinensis (Ohaus, 1914)
12. Adoretus (s str) convexus Burmeister, 1855
13. Adoretus (s str) dilleri Frey, 1970, EAM Frey, 1970
14. Adoretus (s str) fruhstorferi Ohaus, 1905
15. Adoretus (s str) hanoiensis Frey, 1972, EAM Frey, 1972
16. Adoretus (s str) kulzeri Frey, 1967
17. Adoretus (s str) lajoyi Ohaus, 1914, DEZ: 491
18. Adoretus (s str) langsonicus Paulian, 1959
19. Adoretus (s str) luridus Blanchard, 1851 (1850)
20. Adoretus (s str) minutus Brenske, 1893
21. Adoretus (s str) rosettae Frey, 1970, EAM Frey, 1970
22. Adoretus (s str) saigonensis Ohaus, 1914, DEZ: 479
23. Adoretus (s str) sincerus Benderitter, 1929
24. Adoretus (s str) tonkinensis (Ohaus, 1914)
25. Adoretus (s str) vietnamensis Frey, 1970
26. Anomala acutangula Ohaus, 1914
27. Anomala ahrensi Zorn, ZR-2011
28. Anomala amychodes Ohaus, 1914
29. Anomala anchoralis Lansberge, 1879
30. Anomala atriventris Zorn, ZR-2011
31. Anomala bilunata Fairmaire, 1888
32. Anomala bivirgulata Fairmaire, 1893
33. Anomala blaisei Ohaus, 1914
34. Anomala cantorioides Paulian, 1959
35. Anomala castelnaui Ohaus, 1910
36. Anomala cognata Zorn, ZR-2011
37. Anomala curator Benderitter, 1929
38. Anomala dalatensis Frey, 1971
39. Anomala densa Arrow, 1917
40. Anomala edentula Ohaus, 1925
41. Anomala flavofasciata Arrow, 1912
42. Anomala flavoguttata Miyake, 2000
43. Anomala fuscosignata Ohaus, 1905
44. Anomala graminea Ohaus, 1905
45. Anomala gressitti Frey, 1970
46. Anomala hirsutula Nonfried, 1892
47. Anomala holcoptera Fairmaire, 1889
48. Anomala imperialis Arrow, 1899
49. Anomala irideorufa Fairmaire, 1893
50. Anomala iridicollis Ohaus, 1914
51. Anomala itoi Miyake, 1994
52. Anomala jeanvoinei Benderitter, 1929
53. Anomala katsurai Miyake, 1996
54. Anomala kintaroi Miyake, 1996 (new name for babai Miyake, 1994)
55. Anomala langbianensis Zorn, 2011
56. Anomala luminosa Benderitter, 1929
57. Anomala malaisei Paulian, 1959
58. Anomala mausonica Zoen, 2011
59. Anomala millingeni Paulian, 1959
60. Anomala nervulata Paulian, 1959
61. Anomala nigroscutellata Benderitter, 1929
62. Anomala nubeculosa Ohaus, 1905
63. Anomala ovalis Burmeister, 1844
64. Anomala parallela Benderitter, 1929
65. Anomala planelytra Paulian, 1959
66. Anomala platypyga Fairmaire, 1893
67. Anomala praeclara Paulian, 1959
68. Anomala praecoxalis Ohaus, 1914
69. Anomala punctulicollis Fairmaire, 1893
70. Anomala raui Ohaus, 1914
71. Anomala rotundata Paulian, 1959
72. Anomala russiventris Fairmaire, 1893
73. Anomala sapa Miyake, 1994
74. Anomala siamensis Nonfried, 1891
75. Anomala sinica Arrow, 1915
76. Anomala spilopteroides Ohaus, 1914
77. Anomala trillesi Paulian, 1959
78. Anomala truncata ssp. chlorochelys Arrow, 1912
79. Anomala vaga Benderitter, 1929
80. Anomala vietnamica Miyake, 1996
81. Anomala viridimicans Benderitter, 1929
82. Anomala viridisericea Ohaus, 1905
83. Anomala vitalisi Ohaus, 1914
84. Anomalacra clypealis (Schaefer, 1907)
85. Callistethus glandulicollis (Ohaus, 1914)
86. Callistethus metallicus (Benderitter, 1923)
87. Callistethus nuptus (Ohaus, 1905)
88. Callistethus rosetti (Nonfried, 1890)
89. Dicaulocephalus antilocapra Bezdek & Pacholàtko, 2001
90. Dicaulocephalus fruhstorferi Felsche, 1901
91. Dicaulocephalus lamdongensis Muramoto, 2013
92. Didrepanephorus arnaudi Muramoto, 2003
93. Didrepanephorus mizunumai Nagai et H. Hirasawa, 1991
94. Didrepanephorus subvittatus Benderitter, 1929
95. Didrepanephorus takuyai (Muramoto, 2003)
96. Didrepanephorus yunnanus ssp. clermonti Benderitter, 1929
97. Didrepanephorus yunnanus ssp. piaoacensis (Nagai, 2004)
98. Didrepanephorus zen Muramoto, 2009
99. Fruhstorferia anthracina Ohaus, 1903
100. Fruhstorferia egregia Pouillaude, 1915
101. Glenopopillia nagaii (Sabatinelli, 1997)
102. Kibakoganea canhcamsung Do, 2013
103. Kibakoganea fujiokai Miyake & Muramoto, 1992
104. Kibakoganea fujiokai ssp. itohi Muramoto, 2005
105. Kibakoganea kraatzi Miyake et Muramoto, 2003
106. Kibakoganea opaca Muramoto, 1993
107. Kibakoganea sexmaculata (Kraatz, 1900)
108. Kibakoganea tamdaoensis Miyake & Muramoto, 1992
109. Kibakoganea vernicata (Benderitter, 1929)
110. Kibakoganea vernicata ssp. iwasei Muramoto, 1997
111. Lutera nigromaculata Ohaus, 1900
112. Mimela abdominalis Ohaus, 1902
113. Mimela bidentata Lin, 1966
114. Mimela coxalis Ohaus, 1902
115. Mimela chinensis Kirby, 1823
116. Mimela deretzi Paulian, 1959
117. Mimela despumata Ohaus, 1915
118. Mimela epipleurica Ohaus, 1930
119. Mimela foveola Benderitter, 1929
120. Mimela fruhstorferi Ohaus, 1902
121. Mimela heterochropus Blanchard, 1851 (1850)
122. Mimela ignistriata Lin, 1990
123. Mimela nigritarsis Lin, 1990
124. Mimela ngoclinhensis Huong & Wada, 2006
125. Mimela opalina Ohaus, 1902
126. Mimela signaticollis Ohaus, 1902
127. Mimela specularis Ohaus, 1902
128. Mimela xanthorrhoea Ohaus, 1902
129. Parastasia birmana Arrow, 1899
130. Parastasia dalatina Kuijiten, 1992
131. Parastasia hitomi Wada, 1999
132. Parastasia indica Ohaus, 1898
133. Parastasia oberthueri Ohaus, 1900
134. Parastasia rufopicta Westwood, 1842
135. Parastasia sawadai Wada, 2003
136. Parastasia vietnamensis Wada, 2008
137. Peperonota vitalisi Arrow, 1921
138. Popillia amabilis Arrow, 1913
139. Popillia flavofasciata Kraatz, 1892
140. Popillia laevicollis Kraatz, 1892 (homonym of laevicollis Kraatz, p. 229)
141. Popillia ricchiardii Sabatinelli, 1996
142. Popillia uhligi Sabatinelli, 1996
143. Pseudosinghala callosa (Fairmaire, 1888)
144. Pseudosinghala transversa (Burmeister, 1855)
145. Pukupuku katsurai (Muramoto, 2002)
146. Phyllopertha carinicollis Ohaus, 1905
147. Rutelarcha bakeri Ohaus, 1915
148. Spilopopillia tonkinensis Ohaus, 1908
149. Spinanomala benderitteri Burgeon, 1932
150. Trichanomala fossulata (Benderitter, 1929)

Kibakoganea Nagai, 1984

The genus Kikobaganea was erected by Nagai in 1984 as subgenus of Fruhstoferia, Hirasawa promoted it to genus level in 1992. Their typical characteristic is the inner lobe on basal lateral edge of each pronotum side. There are total 14 known species around South East Asia and South China. And they belong to 3 groups (based on structure of the male genitalia organ):


Group 1 with very short and apex separated parameres:

K. formosana (Kurosawa & Kobayashi, 1975) (Do Collection)

This species is endemic in Taiwan, they are quite smaller than other member of the genus, they are green/yellow with black part marking beetles.

K. sinica Bouchard

K. kraatzi Miyake & Muramoto, 2003
This first known from Vietnam then later found from South China and Hainan, the species had been misidentified with K. sexmaculata in a long time but later be described as a new species.

K. opaca Muramoto, 1993 (Do Collection)

This species found from China and recently be recorded from North Vietnam.

Group 2 with longer and not separated parameres which are asymmetrical:

K. kawaii Muramoto, 2005

K. koyamai (Hirasawa, 1989) (Do Collection)

K. kumei (Hirasawa, 1989) (Do Collection)

Last group, group 3, genitalia with not separated and symmetrical parameres:

K. dohertyi (Ohaus, 1905)

K. fujiokai Miyake & Muramoto, 1992 (Do Collection) - Male

Male of K. fujiokai, photographed by Cuong Do, Lam Dong Province

K. fujiokai Miyake & Muramoto, 1992 Female, laying her egg in breeding box. Specimens collected from Lam Dong Privnce

K. sexmaculata (Kraatz, 1900) (Do Collection)
This species is the most early known species among members of the genus. It was first recorded from North Vietnam then be found wider distribute in the area later. The type locality of the species is same to K. kraatzi

K. tamdaoensis Miyake & Muramoto
K. vernicata (Benderitter)

K. canhcamsung Do, 2013 (male holotype) from Tay Giang, Quang Nam Province

K. yoshitomii Nagai

Didrepanephorus Wood Mason, 1878

Didrepnaephorus is the most diversity genus among horned rutelinae, there are total 16 known species of the genus.

Didrepanephorus arnaudi Muramoto, 2003 found from Kon Tum South Vietnam (Do Collection), characteristic with 2 yellow hair stuffs on pronotum dorsally.

Didrepanephorus bifurcifer Wood-Mason, 1878
Didrepanephorus birmanicus (Arrow, 1907)
Didrepanephorus fukinukii (Muramoto & Araya, 2000)

Didrepanephorus lamdongensis Muramoto, 2013 (paratype in Do Collection), this species is new described from Lam Dong and Khanh Hoa Province, South Vietnam in 2013.

Didrepanephorus lao Nagai, 2005
Didrepanephorus malayanus (Nagai & Hirasawa, 1991)

Didrepanephorus mizunumai Nagai & Hirasawa, 1991 (Do Collection)

Didrepanephorus mucronatus Arrow, 1921
Didrepanephorus nishiyamai Muramoto, 2006
Didrepanephorus ohbayashii (Nagai, 2004)

Didrepanephorus pilosus Bouchard, 2007 (Do Collection), known only from Laos

Didrepanephorus subvittatus Benderitter, 1929

Didrepanephorus takuyai (Muramoto, 2003) an endemic species from South Center of Vietnam.

The species D. yunnanus currently consist of 5 subspecies, however I think they should be upgraded to be species levels because they are quite difference each other base on their structure of male genitalia organ.

Didrepanephorus yunnanus clermonti Benderitter, 1929 (Do Collection)

Didrepanephorus yunnanus kachinensis Muramoto, 2005
Didrepanephorus yunnanus piaoacensis (Nagai, 2004)
Didrepanephorus yunnanus wakaharai (Nagai, 2004)


Didrepanephorus yunnanus yunnannus (Ohaus, 1911) (Do Collection)

Didrepanephorus zen Muramoto, 2009 is known as an endemic species of the center of Vietnam.